Primer length min Primer length max Preferred length Tm min Tm max Preferred Tm Max Tm difference GC min GC max Preferred GC

Product size min Product size max Preferred product size Results to show

Na⁺ K⁺ Mg²⁺ dNTPs Primer concentration

Max homopolymer run Max dinucleotide repeat Max G/C in last 5 bases

Hairpin ΔG threshold Self-dimer ΔG threshold Cross-dimer ΔG threshold 3'-end dimer ΔG threshold Candidate pool size Pairs to screen for dimers